anti cyclin e2 rabbit polyclonal cell signaling  (Cell Signaling Technology Inc)

Journal: Molecular and cellular endocrinology

Article Title: DUAL INHIBITION OF ERK1/2 AND AKT PATHWAYS IS REQUIRED TO SUPPRESS THE GROWTH AND SURVIVAL OF ENDOMETRIOTIC CELLS AND LESIONS

doi: 10.1016/j.mce.2018.12.011

Figure Lengend Snippet: Details of the antibody used.

Article Snippet: Anti-human rabbit polyclonal Cyclin E2 , Cell Signaling , 4132 , 1:1000.

Techniques: Concentration Assay

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Motor neuron and pancreas homeobox 1/HLXB9 promotes sustained proliferation in bladder cancer by upregulating CCNE1/2

doi: 10.1186/s13046-018-0829-9

Figure Lengend Snippet: MNX1 directly upregulates CCNE1 and CCNE2 promoter activity in bladder cancer cells. a Real-time PCR of cell cycle–related gene mRNA expression. Gene expression levels were normalized to GAPDH . Pseudo-colors represent the intensity scale of MNX1 versus vector or MNX1 shRNA#1/2 versus Scramble, generated by log2 transformation. b Western blotting of CCNE1, CCNE2, phosphorylated Rb (p-Rb), and total Rb protein expression; α-tubulin was used as the loading control. c The scatter diagram of CCNE1 and CCNE2 from cBioportal program. d and e Left: Luciferase activity assays of T24 and 5637 cells showed transactivation and repression of the CCNE1 ( d ) and CCNE2 ( e ) promoters by MNX1 overexpression and MNX1 knockdown, respectively. Right: Schematic illustration of ChIP PCR fragments for the indicated nucleotide regions of the CCNE1 ( d ) and CCNE2 ( e ) promoters (top). The ChIP enrichment assay confirmed that MNX1 binds to the P4 and P5 promoter of CCNE1 ( d ) and the P6 and P7 promoters of CCNE2 ( e ); immunoglobulin G (IgG) was used as the negative control. The results from three independent experiments were evaluated. * p < 0.05; ** p < 0.01. shRNA, short hairpin RNA

Article Snippet: An anti-MNX1 rabbit polyclonal antibody (1:500 dilution; Sigma Aldrich), an anti-CCNE1 Rabbit polyclonal antibody (1:1000 dilution;Proteintech), an anti-CCNE2 Rabbit polyclonal antibody (1:1000 dilution;Proteintech), an anti-α-tubulin mouse monoclonal antibody (1:4000 dilution; Sigma-Aldrich), an anti-Rb rabbit polyclonal antibody (1:1000 dilution; Cell Signaling Technology), an anti-p-Rb Rabbit polyclonal antibody (1:1000 dilution; Cell Signaling Technology), were used in this study.

Techniques: Activity Assay, Real-time Polymerase Chain Reaction, Expressing, Gene Expression, Plasmid Preparation, shRNA, Generated, Transformation Assay, Western Blot, Control, Luciferase, Over Expression, Knockdown, Negative Control